Thermo Fisher Scientific STUB1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

-

View 2 publications 2 publications

Neutralization (Neu)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rabbit, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues V(218) D E K R K K R D I P D Y L C(232) of human CHIP. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:STUB1,uniProtId:Q9UNE7-1,ncbiNodeId:9606,antigenRange:219-232,antigenLength:303,antigenImageFileName:PA1-015_STUB1_Q9UNE7-1_Rabbit.svg,antigenImageFileNamePDP:PA1-015_STUB1_Q9UNE7-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2271290

Product Specific Information

PA1-015 detects human and mouse carboxyl terminus of hsc70-interacting protein (CHIP).

PA1-015 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~35 kDa protein representing CHIP from COS-1 cells overexpressing the human gene and a non-specific band at ~85 kDa.

PA1-015 was used successfully in the western blot analysis of CHIP using human MCF7 cell lysates.

The PA1-015 immunogen is a synthetic peptide corresponding to residues V(218) D E K R K K R D I P D Y L C(232) of human CHIP. This peptide (Cat. # PEP-179) is available for use in neutralization and control experiments.

Target Information

A recently identified protein, termed carboxyl terminus of hsc70-interacting protein (CHIP), has been shown to interact both with the constitutive form of hsc70 and the stress inducible form, hsp70. This novel 35 kDa cytoplasmic protein has been shown to be highly expressed in striated muscle in vivo. Additional studies have shown that this protein is expressed over a broad range of cultured tissues. Through immunoprecipitation experiments, CHIP has been shown to directly bind to the carboxyl terminus of hsc70 and hsp70 where it decreases ATPase activity and reduces overall chaperone efficiency. CHIP has also been identified as an important protein in the ubiquitin-proteasome system. CHIP contains a U-box domain and acts as an E3 ubiquitin-ligase in conjunction with hsc70 and hsp90.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.