
Thermo Fisher Scientific CXCL3 Polyclonal Antibody, Biotin, PeproTech
Human CXCL3(GRO-gamma)에 특이적인 Biotin 표지 폴리클로날 항체로, Western blot 및 ELISA에 적합. Rabbit 유래, 항원 친화 크로마토그래피로 정제됨. 고감도 검출 및 안정적 보관(-20°C) 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 0.1–0.2 µg/mL
ELISA
- Tested Dilution: 0.25–1.0 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human GRO-gamma (CXCL3) |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | No preservative |
| Storage conditions | -20°C |
| Shipping conditions | Ambient |
| RRID | AB_2929214 |
Product Specific Information
AA Sequence of recombinant protein:
ASVVTELRCQ CLQTLQGIHL KNIQSVNVRS PGPHCAQTEV IATLKNGKKA CLNPASPMVQ KIIEKILNKG STN
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human GRO-gamma (CXCL3). Anti-Human GRO-gamma (CXCL3)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect Human GRO-gamma by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human GRO-gamma (500-P105) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human GRO-gamma.
Western Blot:
To detect Human GRO-gamma by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human GRO-gamma is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Packaging:
500-P105BT-1MG is provided as 2 × 500 µg.
Target Information
CXCL3 may play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. All three isoforms of GRO are CXC chemokines that can signal through the CXCR1 or CXCR2 receptors. The GRO proteins chemoattract and activate neutrophils and basophils.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일명: 500-P105BT-25UG_CXCL3_P19876-1_Rabbit.svg, 500-P105BT-25UG_CXCL3_P19876-1_Rabbit_PDP.jpeg)
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