Thermo Fisher Scientific CXCL3 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
ASVVTELRCQ CLQTLQGIHL KNIQSVNVRS PGPHCAQTEV IATLKNGKKA CLNPASPMVQ KIIEKILNKG STN

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human GRO-gamma (CXCL3). Anti-Human GRO-gamma (CXCL3)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human GRO-gamma by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human GRO-gamma (500-P105) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human GRO-gamma.

Western Blot:
To detect Human GRO-gamma by Western Blot, use at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human GRO-gamma is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P105BT-1MG is provided as 2 × 500 µg.

Target Information

CXCL3 may play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. All three isoforms of GRO are CXC chemokines that can signal through the CXCR1 or CXCR2 receptors. The GRO proteins chemoattract and activate neutrophils and basophils.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.