Thermo Fisher Scientific CCL14 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
TESSSRGPYH PSECCFTYTT YKIPRQRIMD YYETNSQCSK PGIVFITKRG HSVCTNPSDK WVQDYIKDMK EN

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human HCC-1 (CCL14). Anti-Human HCC-1 (CCL14)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human HCC-1 (CCL14) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human HCC-1 (CCL14) (500-P106) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human HCC-1 (CCL14).

Western Blot:
To detect hHCC-1 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hHCC-1 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Packaging:
500-P106BT-1MG will be provided as 2 × 500 µg.

Target Information

The CCL14 gene is one of several CC cytokine genes clustered on chromosome 17q11.2. CC cytokines are secreted proteins characterized by two adjacent cysteines. The cytokine encoded by this gene induces changes in intracellular calcium concentration and enzyme release in monocytes. This gene expresses both monocistronic and bicistronic transcripts, with bicistronic transcripts including the upstream cytokine gene CCL15. CCL14 undergoes alternative splicing in bicistronic transcripts; it is unknown if its monocistronic transcript is also subject to alternative splicing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.