
Thermo Fisher Scientific CXCL2 Polyclonal Antibody, Biotin, PeproTech
CXCL2(GRO-beta)에 특이적인 토끼 폴리클로날 항체로, 비오틴으로 표지되어 ELISA 및 Western blot 분석에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, -20°C에서 보관합니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific CXCL2 Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| ELISA | 0.25–1.0 µg/mL | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived Recombinant Human GRO-beta (CXCL2) |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
Product Specific Information
AA Sequence of recombinant protein:APLATELRCQ CLQTLQGIHL KNIQSVKVKS PGPHCAQTEV IATLKNGQKA CLNPASPMVK KIIEKMLKNG KSN
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human GRO-beta (CXCL2). Anti-Human GRO-beta (CXCL2)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect Human GRO-beta (CXCL2) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human GRO-beta (CXCL2) (500-P104) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human GRO-beta (CXCL2).
Western Blot:
To detect Human GRO-beta by Western blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human GRO-beta is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Packaging:
500-P104BT-1MG is provided as 2 × 500 µg.
Target Information
Produced by activated monocytes and neutrophils and expressed at sites of inflammation. GRO-beta acts as a hematoregulatory chemokine that suppresses hematopoietic progenitor cell proliferation in vitro. GRO-beta(5-73) shows highly enhanced hematopoietic activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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