Thermo Fisher Scientific MCM7 Recombinant Mouse Monoclonal Antibody (rMCM7, 1468)
rMCM7 단백질을 인식하는 Thermo Fisher Scientific의 재조합 마우스 단일클론 항체로, WB 및 IHC(P)에서 1–2 µg/mL 농도로 사용 가능. HEK293 세포에서 발현되었으며 Protein A/G로 정제됨. PBS 기반 버퍼에 보관하며 4°C에서 안정적.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rMCM7, 1468 |
| Immunogen | Recombinant human MCM7 protein fragment (aa195–319) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
MCM7 encodes a protein that is one of the highly conserved mini-chromosome maintenance (MCM) proteins essential for the initiation of eukaryotic genome replication.
The hexameric complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in replication fork formation and recruitment of other DNA replication-related proteins.
The MCM complex consisting of MCM7 with MCM2, 4, and 6 possesses DNA helicase activity, functioning as a DNA unwinding enzyme. Cyclin D1-dependent kinase CDK4 associates with MCM7 and may regulate its interaction with the tumor suppressor protein RB1/RB.
Alternatively spliced transcript variants encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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