Thermo Fisher Scientific MCM7/CDC47 (Proliferation Marker) Monoclonal Antibody (SPM379)
MCM7/CDC47 단백질을 표적하는 단클론 항체로 세포 증식 마커 연구에 적합함. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용 가능. 인간 반응성, Mouse IgG1, kappa 아이소타입. 4°C 보관, Protein A/G 정제, 연구용 전용.
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Thermo Fisher Scientific MCM7/CDC47 (Proliferation Marker) Monoclonal Antibody (SPM379)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SPM379 |
| Immunogen | Recombinant human hCDC47 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
MCM7 encodes a protein that is one of the highly conserved mini-chromosome maintenance proteins (MCM) essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in replication fork formation and recruitment of other DNA replication-related proteins. The MCM complex (including MCM2, 4, and 6) possesses DNA helicase activity and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase CDK4 associates with this protein and may regulate its interaction with the tumor suppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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