Thermo Fisher Scientific MCM7 Recombinant Mouse Monoclonal Antibody (rMCM7, 1468)
Recombinant mouse monoclonal antibody targeting MCM7 protein. Validated for WB, IHC, and peptide array applications. High purity, unconjugated, and protein A/G purified. Suitable for research use in eukaryotic DNA replication studies.
- 카탈로그번호
- 4176-MSM9-P1ABX
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific MCM7 Recombinant Mouse Monoclonal Antibody (rMCM7, 1468)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Dog, Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rMCM7, 1468 |
| Immunogen | Recombinant human MCM7 protein fragment (aa195–319) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
MCM7 encodes a highly conserved mini-chromosome maintenance protein essential for the initiation of eukaryotic genome replication. The MCM proteins form a hexameric complex that is a key component of the pre-replication complex (pre_RC) and play roles in replication fork formation and recruitment of DNA replication-related proteins.
The complex consisting of MCM7 with MCM2, MCM4, and MCM6 possesses DNA helicase activity and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, associates with MCM7 and may regulate its interaction with the tumor suppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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