Thermo Fisher Scientific MCM7 Recombinant Rabbit Monoclonal Antibody (MCM7, 2756R)
Recombinant rabbit monoclonal antibody targeting human MCM7 for WB and IHC applications. High purity Protein A/G purified, liquid form at 200 µg/mL. Suitable for detecting MCM7 in replication studies. For research use only.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC-P) | 1–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | MCM7, 2756R |
| Immunogen | Recombinant fragment of human MCM7 (aa195–319) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
MCM7 encodes a protein that is one of the highly conserved mini-chromosome maintenance (MCM) proteins essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and recruitment of other DNA replication-related proteins.
The MCM complex consisting of MCM2, MCM4, MCM6, and MCM7 possesses DNA helicase activity and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, associates with MCM7 and may regulate its binding with the tumor suppressor protein RB1/RB.
Alternatively spliced transcript variants encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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