Thermo Fisher Scientific ACVR2A Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

15 µg/mL

-

ELISA (ELISA)

0.5-1 µg/mL

-

Product Specifications

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment of human Activin Receptor Type IIA.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2222297

Product Specific Information

Recommended positive controls: muscle fibroblasts.

Neutralization: when used at a 1-3 µg/mL, the antibody will block 50% of the binding of 30 ng/mL recombinant human activin A to immobilized recombinant human activin receptor IIA/Fc chimera (100 µL of a 0.5 µg/mL solution coating each well) in a functional ELISA assay.

Target Information

Activin A receptor kinase (ACVR2A) is a type II member of the TGF-beta family of receptor Serine/Threonine kinases. ACVR2A is involved in the activin and myostatin signaling pathway. (ACVR2A Aliases: ACVR2, ACTRII). These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling, and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Type II receptors are considered to be constitutively active kinases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.