Thermo Fisher Scientific MEK2 N-Term Monoclonal Antibody (19G10.F1.E2), DyLight 800
MEK2 N-Term 특이적 Monoclonal Antibody로 DyLight 800 형광 표지. Human, Mouse, Rat 반응성. Western blot 및 ELISA 검증 완료. 단백질 A 정제 및 안정적 보관 가능. 연구용으로 신호전달 경로 분석에 적합.
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Thermo Fisher Scientific MEK2 N-Term Monoclonal Antibody (19G10.F1.E2), DyLight 800
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| ELISA | 1:40,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 19G10.F1.E2 |
| Immunogen | Synthetic peptide corresponding to amino acid residues near the N-terminus conjugated to KLH |
| Conjugate | DyLight™ 800 |
| Excitation / Emission Max | 789 / 794 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Reacts specifically with human MEK2. Cross-reactivity is expected with human, mouse, and rat based on sequence identity of the peptide immunogen. This antibody does not react with the MEK1 isoform.
Target Information
MAP2K2 (MEK2) is a dual specificity serine/threonine kinase involved in mitogen growth factor signal transduction. MEK2 (MAPKK2) belongs to a family of tyrosine/threonine kinases that activate ERK1/2 and MAPK enzymes by phosphorylating residues within the TEY motif in the activation loop. MEK1 and MEK2 are activated by dual phosphorylation on serine 218 and 222. Threonine 292 of MEK1 is phosphorylated by ERK2, functioning as a negative feedback mechanism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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