Thermo Fisher Scientific 6X His Tag Monoclonal Antibody (33D10.D2.G8), DyLight 405
6X His 태그 단백질 검출용 단일클론 항체로 DyLight 405 형광 표지. Western blot, ICC/IF, ELISA 등 다양한 응용에 적합. 높은 특이성과 비특이적 반응 최소화. 단기 4°C, 장기 -20°C 보관 권장.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | >1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 33D10.D2.G8 |
| Immunogen | This 6X HIS antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide. |
| Conjugate | DyLight™ 405 |
| Excitation / Emission Max | 400 / 423 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
- Unconjugated
- Atto 550
- Atto 647N
- Biotin
- Cyanine3
- Cyanine5
- DyLight 488
- DyLight 549
- DyLight 649
- DyLight 680
- DyLight 800
- HRP
- Request custom conjugation
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze/thaw cycles. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
6X His Tag Antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This monoclonal antibody detects overexpressed proteins containing the 6X His epitope tag. It has shown reactivity with all His-tagged proteins tested. In western blotting of bacterial extracts, it does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH). It is commonly expressed at either N- or C-terminal regions of recombinant proteins to facilitate isolation or purification by immobilized metal affinity chromatography (IMAC). Epitope tagging enables detection of recombinant proteins for which no specific antibody exists. His-tagged proteins are purified based on the strong and selective affinity between histidine residues and metal ions such as Ni²⁺ on Ni²⁺-NTA adsorbents. His tag-specific antibodies are widely used for detection or co-immunoprecipitation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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