Thermo Fisher Scientific ICAD Polyclonal Antibody
ICAD 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, IHC(P)에서 1:100 희석 비율로 사용됩니다. 인간 DFF-45 N-말단 유래 합성 펩타이드로 면역화되었으며, 비결합형 액상 형태입니다. 항원 회복을 위해 10mM 시트르산 완충액(pH 6.0)을 권장합니다.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from N-terminus of human DFF-45 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2092624 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
A suggested positive control is colon carcinoma.
Target Information
ICAD is the inhibitor of caspase-3-activated DNase which controls nuclear apoptosis.
It has two isoforms:
- ICAD-L/DFF45 (45 kDa) – functional isoform
- ICAD-S/DFF35 (35 kDa) – non-functional isoform
Only ICAD-L is functional and inhibits CAD until cleaved by caspase-3 at residues 117 and 224, releasing three fragments.
ICAD also acts as a CAD-specific folding chaperone.
ICAD may be a potential target for restoring normal apoptotic signaling in colon and brain cancer cells.
Two alternatively spliced transcript variants encoding distinct isoforms have been identified for the ICAD gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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