Thermo Fisher Scientific DJ-1 Polyclonal Antibody
Rabbit polyclonal antibody against human DJ-1 protein for WB and IHC applications. Recognizes multiple species based on sequence homology. Suitable for oxidative stress and Parkinson’s disease research. Provided in liquid form, stored at 4°C or -20°C.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:50 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from C-terminus of human DJ-1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_1077461 |
Product Specific Information
This antibody is predicted to react with bovine, chicken, canine, mouse, porcine, and rat based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10–20 minutes followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
A suggested positive control is normal breast tissue.
Target Information
The DJ-1 gene product belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription and may function as a redox-sensitive chaperone and oxidative stress sensor. It protects neurons against oxidative stress and cell death.
Defects in this gene cause autosomal recessive early-onset Parkinson disease 7.
Two transcript variants encoding the same protein have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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