Thermo Fisher Scientific Cyclin H Recombinant Rabbit Monoclonal Antibody (SN20-48)
Cyclin H 단백질을 인식하는 재조합 토끼 단클론 항체로, 높은 특이성과 민감도를 제공합니다. Western blot, IHC, ICC/IF, Flow 등 다양한 응용에 사용 가능하며, HEK293 세포 발현 시스템으로 생산되었습니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 | View 1 publication |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 | – |
| Flow Cytometry (Flow) | 1:50–1:100 | – |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SN20-48 |
| Immunogen | Recombinant protein within Human Cyclin H (aa 230–323) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809612 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages include:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Cyclin H belongs to the highly conserved cyclin family, characterized by periodic changes in protein abundance through the cell cycle. Cyclins regulate CDK kinases and exhibit distinct expression and degradation patterns, coordinating mitotic events.
Cyclin H forms a complex with CDK7 kinase and MAT1, functioning as a CDK-activating kinase (CAK). This complex phosphorylates CDK2 and CDC2 kinases and is part of TFIIH and RNA polymerase II complexes, linking transcriptional regulation with cell cycle control.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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(이미지 파일명: MA5-32331_Cyclin_H_P51946-1_Rabbit.svg, MA5-32331_Cyclin_H_P51946-1_Rabbit_PDP.jpeg)
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