Thermo Fisher Scientific H3K79ac Recombinant Rabbit Monoclonal Antibody (RM156)
Histone H3의 Lys79 아세틸화(K79ac)에 특이적으로 반응하는 재조합 토끼 단클론 항체. Western blot, ICC/IF, ELISA, ChIP, Luminex 등 다양한 응용에 사용 가능. 높은 특이성과 일관된 로트 간 재현성 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–5 µg/mL |
| ELISA | 0.2–1 µg/mL |
| ChIP assay (ChIP) | 1.5 µg / 1×10⁶ cells |
| Luminex (LUM) | 0.05–0.2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM156 |
| Immunogen | Acetyl-peptide corresponding to the Acetyl-Histone H3 (Lys79) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2–7.4, with 50% glycerol, 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2661905 |
Product Specific Information
This antibody reacts specifically with Histone H3 acetylated at Lysine 79 (K79ac) and shows no cross-reactivity with other acetylated lysines in Histone H3.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, followed by screening to select optimal clones for production.
Advantages:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to rabbit immune repertoire
Target Information
Histone H3 is one of the core DNA-binding proteins found in chromatin of eukaryotic cells. It forms nucleosomes with other histones to help organize DNA. Histones are central to transcription regulation, DNA repair, replication, and chromosomal stability. The N-terminal tail of histone H3 can undergo multiple post-translational modifications—such as methylation, acetylation, and phosphorylation—that influence chromatin structure and gene expression.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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