Thermo Fisher Scientific H3K23me2 Recombinant Rabbit Monoclonal Antibody (RM171)
Histone H3 Lys23 이중메틸화(K23me2)에 특이적으로 반응하는 재조합 토끼 단클론 항체. Western blot, ICC, ELISA, ChIP 등 다양한 응용에 적합. 높은 특이성과 일관된 로트 간 재현성 제공. -20°C 보관, Protein A 정제.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
| ELISA | 0.1–0.5 µg/mL |
| ChIP assay (ChIP) | 1.5 µg/1×10⁶ cells |
| Luminex (LUM) | 0.1–0.5 µg/mL |
| Dot blot (DB) | 0.5–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM171 |
| Immunogen | Di-methyl-peptide corresponding to Di-methyl-Histone H3 (Lys23) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2–7.4, with 50% glycerol, 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2661911 |
Product Specific Information
This antibody reacts specifically to Histone H3 dimethylated at Lysine 23 (K23me2).
No cross-reactivity observed with monomethylated (K23me1), trimethylated (K23me3), or other methylation sites in histone H3.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning antibody DNA sequences from immunoreactive rabbits.
Advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin–free formulation
- Broader immunoreactivity due to rabbit immune repertoire
Target Information
Histone H3 is a core DNA-binding protein of chromatin in eukaryotic cells.
Together with other histones, it forms nucleosomes, contributing to chromatin structure and regulation of transcription, DNA repair, replication, and stability.
Histone H3 undergoes various post-translational modifications, such as methylation, acetylation, and phosphorylation, which influence chromatin dynamics and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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