Thermo Fisher Scientific H3K36ac Recombinant Rabbit Monoclonal Antibody (RM154), ChIP-Verified
H3K36ac에 특이적인 재조합 토끼 단클론 항체로, ChIP 검증 완료. Western blot, ICC/IF, ELISA 등 다양한 응용에 사용 가능. 높은 특이성과 재현성을 제공하며, 로트 간 일관성과 동물유래 성분이 없는 포뮬레이션.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–2 µg/mL | View 1 publication |
| Immunocytochemistry (ICC/IF) | 0.5–5 µg/mL | - |
| ELISA | 0.2–1 µg/mL | - |
| ChIP assay (ChIP) | 1–5 µg | - |
| Luminex (LUM) | 0.1–0.5 µg/mL | - |
| Peptide Array | 0.25 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Yeast |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM154 |
| Immunogen | Acetyl-peptide corresponding to Acetyl-Histone H3 (Lys36) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2–7.4, with 50% glycerol, 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2661914 |
Product Specific Information
This antibody reacts specifically with Histone H3 acetylated at Lysine 36 (H3K36ac) and is not affected by phosphorylation at neighboring Ser31. It shows no cross-reactivity with other acetylated lysines in histone H3.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Click here for Master Lot linking information
Target Information
Histone H3 is a DNA-binding protein forming part of the chromatin structure in eukaryotic cells. Along with other core histones, it binds DNA to form nucleosomes. Histones play crucial roles in transcription regulation, DNA repair, replication, and chromosomal stability.
The N-terminal tail of histone H3 can undergo multiple post-translational modifications, such as methylation, acetylation, and phosphorylation, which influence gene expression and chromatin dynamics.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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