Thermo Fisher Scientific Histone H3.3 Recombinant Rabbit Monoclonal Antibody (RM190)
Histone H3.3 특이적 재조합 토끼 단클론 항체로, H3.1 및 기타 히스톤과 교차 반응 없음. Western blot, ELISA, ChIP 등 다양한 응용에 적합. 높은 특이성과 재현성을 제공하며, in vitro 발현 시스템으로 생산된 동물 유래 성분 무첨가 항체.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–2 µg/mL | View publication |
| Immunocytochemistry (ICC/IF) | – | View publication |
| ELISA | 0.2–1 µg/mL | – |
| ChIP assay (ChIP) | 1.5 µg/1×10⁶ cells | – |
| Luminex (LUM) | 0.05–0.5 µg/mL | – |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM190 |
| Immunogen | Peptide corresponding to human Histone H3.3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS (pH 7.2–7.4) with 50% glycerol, 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | –20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2661918 |
Product Specific Information
This antibody reacts specifically to Histone H3.3, independent of post-translational modifications.
No cross-reactivity with Histone H3.1 or other histone proteins.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits and screen individual clones to select optimal candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Histones are basic nuclear proteins responsible for nucleosome structure in eukaryotic chromosomal fibers.
Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of the four core histones (H2A, H2B, H3, and H4).
Chromatin fiber is compacted through interaction of linker histone H1 with DNA between nucleosomes, forming higher-order chromatin structures.
The Histone H3.3 gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails and instead contain a palindromic termination element.
This gene is located separately from other H3 genes within the histone gene cluster on chromosome 6p22–p21.3.
For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.
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