Thermo Fisher Scientific H3K27ac Recombinant Rabbit Monoclonal Antibody (RM172)
Histone H3 Lys27 아세틸화에 특이적인 재조합 토끼 단클론 항체. Western blot, ICC/IF, ELISA, Luminex 등 다양한 응용에 적합. 높은 특이성과 재현성을 제공하며 로트 간 일관성 우수. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–5 µg/mL |
| ELISA | 0.2–1 µg/mL |
| Luminex (LUM) | 0.1–0.5 µg/mL |
| Peptide Array | 0.25 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM172 |
| Immunogen | Acetyl-peptide corresponding to Acetyl-Histone H3 (Lys27) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2–7.4, with 50% glycerol, 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2661899 |
Product Specific Information
This antibody reacts specifically with Histone H3 acetylated at Lysine 27 (H3K27ac) and shows no cross-reactivity with other acetylated lysines in histone H3.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning the specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- High specificity and sensitivity
- Excellent lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Together with four core histone proteins, it forms the nucleosome structure that organizes DNA. Histones are key regulators of transcription, DNA repair, replication, and chromosomal stability.
The N-terminal tail of histone H3 can undergo multiple post-translational modifications, including methylation, acetylation, and phosphorylation, which influence chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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