Thermo Fisher Scientific VISTA Chimeric Recombinant Rabbit Monoclonal Antibody (MH5A)
VISTA 단백질을 표적하는 재조합 토끼 단클론 항체로, 다양한 면역학적 분석(IHC, ICC/IF, ELISA 등)에 사용 가능. PD-1H에 특이적으로 결합하며, 암 면역치료 연구에 적합. 고순도 Protein A 정제, 액상 형태로 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| ELISA | 1.7 ng/mL |
| Functional Assay | Assay-dependent |
| Inhibition Assay | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG, lambda |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | MH5A |
| Immunogen | The antibody was generated by immunising Armenian hamsters with mPD-1HIg. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3074569 |
Product Specific Information
This antibody specifically binds PD-1H.
Target Information
VISTA (V-domain Ig containing suppressor of T-cell activation), also known as B7-H5 or PD-1H, is a product of the VSIR gene. Although VISTA bears sequence homology to PD-L1, it has a distinct expression pattern. In both mouse and human, VISTA is predominantly expressed on hematopoietic cells, with the highest densities on myeloid cells and lower levels on T cells. It is typically absent on B cells. VISTA expressed by antigen-presenting cells directly suppresses proliferation and cytokine production of CD4 and CD8 T cells, making it a promising target for cancer immunotherapy. Reported ligands include CD28H, VSIG-3, and VSIG-8.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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