Thermo Fisher Scientific TIMP1 Polyclonal Antibody, Biotin, PeproTech
Biotin으로 표지된 Rabbit Polyclonal Anti-Human TIMP1 항체로, Western Blot 및 ELISA에 최적화됨. 고순도 재조합 단백질로 면역화되어 항원 친화 크로마토그래피로 정제됨. 세포 신호 조절 및 MMP 억제 연구에 적합. 연구용으로만 사용 가능.
- 카탈로그번호
- 500-P280BT-xxxx (2개 옵션)
- 판매단위
- pk
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Thermo Fisher Scientific TIMP1 Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.25–1.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived, 20.6 kDa Recombinant Human TIMP-1 |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930231 |
Product Specific Information
AA Sequence of recombinant protein:
CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQ ALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLQGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human TIMP-1. Anti-Human TIMP-1-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect hTIMP-1 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human TIMP-1 (500-P280) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hTIMP-1.
Western Blot:
To detect hTIMP-1 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hTIMP-1 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Packaging:
500-P280BT-1MG is provided as 2 × 500 µg.
Target Information
TIMP1 (metalloproteinase inhibitor 1) is a metalloproteinase inhibitor that forms one-to-one complexes with target metalloproteinases such as collagenases, irreversibly inactivating them by binding to their catalytic zinc cofactor. TIMP1 acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP14, and MMP16. It also functions as a growth factor regulating cell differentiation, migration, and apoptosis. TIMP1 activates cellular signaling cascades via CD63 and ITGB1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일: 500-P280BT-25UG_TIMP1_P01033-1_Rabbit_PDP.jpeg, 500-P280BT-25UG_TIMP1_P01033-1_Rabbit.svg)
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