Thermo Fisher Scientific MUTYH Polyclonal Antibody
MUTYH 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF 등 다양한 응용 가능. 인간, 마우스, 랫트, 박테리아에 반응하며, 단백질 G로 정제된 액상형 항체. DNA 손상 복구 연구에 적합.
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Thermo Fisher Scientific MUTYH Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2 µg/mL |
| Immunohistochemistry (IHC) | 1:10–1:2,000 |
| Immunocytochemistry (ICC/IF) | 18 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bacteria, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 531–546 of human MYH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2147401 |
Product Specific Information
Suggested positive control: HeLa whole cell extract.
Target Information
MYH (mutY homolog, E. coli) is a DNA glycosylase mismatch repair enzyme that, together with mutM (OGG1), removes adenine residues mispaired with oxidized or normal guanines to correct A/G and A/C mismatches. This process initiates base excision repair to prevent mutations caused by oxidative DNA damage. Multiple N-terminal splice variants of MYH exist in mammals. Elevated MYH levels in A549 cells exposed to oxygen and infrared radiation enhance cell survival. Biallelic MYH germ-line mutations predispose humans to colorectal adenomas and carcinomas. MYH is abundant in neurons, maintaining mitochondrial DNA integrity against reactive oxygen species throughout the mammalian lifespan.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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