Thermo Fisher Scientific CD11b Monoclonal Antibody (ICRF44), Brilliant Ultra Violet 395, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (1.0 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 496 / 563 / 615 / 661 / 737 / 805
• Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
• eFluor 506
• FITC
• NovaFluor Blue, Red, Violet, Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702 / 780
• Custom conjugation available upon request

Product Specific Information

The ICRF44 monoclonal antibody reacts with human CD11b, a 165 kDa adhesion molecule associated with integrin beta2 (CD18). CD11b is expressed on monocytes, granulocytes, activated lymphocytes, and a subset of NK cells. It serves as a receptor for intercellular adhesion molecules CD54, CD102, CD50, and complement component iC3b, playing a key role in cell-cell and cell-matrix interactions.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and validated for flow cytometry at 5 µL (1.0 µg) per 100 µL test volume. Suitable for 10⁵–10⁸ cells/test.

Fluorochrome Information:
Brilliant Ultra Violet™ 395 (BUV395) emits at 395 nm and is optimized for cytometers equipped with a 355 nm UV laser. Ensure instrument compatibility before use.

Staining Recommendations:
When using multiple polymer dye-conjugated antibodies (e.g., Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Cat. SB-4401-42) or Brilliant Stain Buffer™ (Cat. 00-4409-75) to minimize non-specific interactions.

Excitation: 350 nm
Emission: 395 nm
Laser: Ultraviolet (UV)

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen.

Target Information

CD11b (integrin alpha-M, ITGAM) forms a heterodimer with CD18 (integrin beta-2) to create the Mac-1 (CR3) complex. It is expressed on monocytes/macrophages, granulocytes, activated lymphocytes, subsets of NK and dendritic cells, and microglia. CD11b/CD18 functions as a receptor for ICAM family members (CD54, CD102, CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X. Expression varies by cell type and maturation stage, and it is a key marker for microglia in nervous tissue. Altered CD11b function is linked to systemic lupus erythematosus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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