Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Brilliant Ultra Violet 395, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Brilliant Ultra Violet 395, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test

Product Specifications

Product Specific Information

Description:
The 53-6.7 monoclonal antibody reacts with mouse CD8a, a 32–34 kDa cell surface receptor expressed as either a heterodimer (CD8αβ) or homodimer (CD8αα). CD8 interacts with MHC class I and associates with tyrosine kinase p56lck, playing a critical role in T cell development and activation.

Applications Reported:
This antibody is suitable for flow cytometric analysis.

Applications Tested:
Validated by flow cytometric analysis of mouse splenocytes. Recommended usage ≤ 0.5 µg/test (100 µL final volume). Cell number may vary from 10⁵ to 10⁸ cells/test. Optimal titration is advised.

Fluorochrome Information:
Brilliant Ultra Violet™ 395 (BUV395) emits at 395 nm and is optimized for instruments with a 355 nm UV laser.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Excitation / Emission / Laser:
350 nm / 395 nm / Ultraviolet Laser

Trademark Notice:
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family involved in T cell signal transduction, differentiation, and activation. CD8 acts as a co-receptor for MHC class I molecules and is expressed on T cells, NK cells, monocytes, and dendritic cells. Engagement of MHC-I/peptide complexes triggers Lck-mediated activation leading to cytokine production and cytotoxic T lymphocyte (CTL) response. Differentiation of naive CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures.

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 496, 563, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506, 615, 660
• FITC
• Functional Grade
• NovaFluor Blue, Red, Violet, Yellow series
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Custom conjugation available

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