Thermo Fisher Scientific GFP Recombinant Rabbit Monoclonal Antibody (SR48-02)
GFP 단백질을 인식하는 Thermo Fisher Scientific의 재조합 토끼 단클론 항체로, WB, IHC, ICC, IP 등 다양한 응용에 적합. 높은 특이성과 재현성을 제공하며, HEK293 세포에서 발현된 IgG 형 항체. 연구용으로만 사용 가능.
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Thermo Fisher Scientific GFP Recombinant Rabbit Monoclonal Antibody (SR48-02)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SR48-02 |
| Immunogen | Synthetic peptide within Aequorea victoria GFP aa 1–50/238 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at −20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2931492 |
Target Information
The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in its bioluminescence reaction. GFP is widely used as a reporter protein for gene expression in both eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular systems. As a fusion tag, GFP enables localization studies, tracking of protein movement, and analysis of subcellular compartment dynamics.
GFP is a 27 kDa monomeric protein that forms a fluorescent pigment autocatalytically. The wild-type protein absorbs blue light (max 395 nm) and emits green light (peak 508 nm) without requiring additional proteins or cofactors. Its fluorescence is stable and species-independent, making it suitable for diverse applications such as monitoring gene expression, protein localization, studying protein-protein interactions (yeast two-hybrid system), and measuring molecular distances via fluorescence energy transfer (FRET).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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