Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (3D12), PerCP-eFluor 710, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.06 µg)/test
• Publications: View 11 publications

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395, 563, 661, 737
• Brilliant Violet 421, 480, 711, 786
• FITC
• NovaFluor Red 685, 700, 755
• NovaFluor Yellow 700
• PE, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• Super Bright 600, 645, 780
• Request custom conjugation

Product Specific Information

Description:
The 3D12 monoclonal antibody reacts with human CCR7 (CD197), a G-protein-coupled chemokine receptor with seven transmembrane domains. CCR7 acts as a receptor for 6Ckine/SLC (CCL19 and CCL21) and is expressed on T cells, distinguishing naïve, central, and effector memory subsets. It also plays a key role in memory T cell migration to inflamed tissues and is expressed on dendritic cells (DCs), where expression increases during maturation, contributing to chemotaxis, endocytosis, and survival.

Applications Reported:

• Flow cytometric analysis.

Applications Tested:

• Pre-titrated and validated for flow cytometry of normal human peripheral blood cells.
• Recommended use: 5 µL (0.06 µg) per test in 100 µL final volume.
• Suitable for 10⁵–10⁸ cells/test.
• Optimal staining: incubate ≥45 minutes at 2–8°C.

Fluorochrome Information:

• PerCP-eFluor® 710 can replace PE-Cy5, PE-Cy5.5, or PerCP-Cy5.5.
• Excitation: 488 nm (Blue laser)
• Emission: 710 nm
• Recommended filters: 685 LP dichroic mirror and 710/40 band pass (695/40 acceptable).
• Stable in 2% formaldehyde overnight at 4°C.
• Filtered post-manufacturing (0.2 µm).

Target Information

CCR7 is a G protein-coupled chemokine receptor induced by Epstein-Barr virus (EBV). It mediates EBV effects on B lymphocytes and is expressed in blood, bone marrow, lymph nodes, and intestines. CCR7 regulates memory T cell migration to inflamed tissues and promotes dendritic cell maturation. Its specific ligands include CCL19 (ECL) and CCL21. Related C-C chemokine receptors include CCR1–CCR5 and the Duffy antigen, all contributing to immune cell chemotaxis and inflammation response.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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