Thermo Fisher Scientific Ki-67 Monoclonal Antibody (MKI67, 2461)
Ki-67 단백질을 인식하는 마우스 단클론 항체로, 세포 증식 지표 연구에 활용. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용 가능. Human 시료에 반응하며, 단백질 A/G로 정제된 액상 형태. 냉동(-20°C 이하) 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MKI67, 2461 |
| Immunogen | Recombinant fragment of human Ki67 protein (around aa 2293–2478) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | –20°C or –80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA-fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 min.
Target Information
Ki-67 is a nuclear protein expressed during various stages of the cell cycle, particularly in late G1, S, G2, and M phases.
It contains a forkhead-associated (FHA) domain that mediates association with euchromatin at the perichromosomal layer, centromeric heterochromatin, and nucleolus.
Ki-67 shows a cell cycle-dependent localization: perinucleolar expression in G1, nuclear matrix localization in G2, and chromosomal association during M phase.
It serves as a proliferation marker since it is absent in G0 cells but increases steadily from G1 through mitosis.
Ki-67 antibodies are widely used to determine the proliferative fraction in neoplasms, with strong correlation between low Ki-67 index and low-grade tumors.
Commonly used as a neuronal marker for cell cycling and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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