Thermo Fisher Scientific Ki-67 (Proliferating Cell Marker) Monoclonal Antibody (MKI67/6582)
인간 Ki-67 단백질을 표적하는 단클론 항체로, 세포 증식 마커 분석에 적합합니다. Western blot, IHC, Flow cytometry 등 다양한 응용 가능. 핵 내 위치하며, 증식 중인 세포의 양적 평가에 유용. 연구용으로만 사용.
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Thermo Fisher Scientific Ki-67 (Proliferating Cell Marker) Monoclonal Antibody (MKI67/6582)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2–4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MKI67/6582 |
| Immunogen | Recombinant human Ki67 protein fragment (around aa 2293–2478) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive Control: Any actively proliferating cells such as human skin, tonsil, or lymph node.
- Cellular Location: Nucleus.
Target Information
Ki-67 is a nuclear protein expressed during various stages of the cell cycle, particularly during late G1, S, G2, and M phases. It contains a forkhead-associated (FHA) domain that mediates association with euchromatin at the perichromosomal layer, centromeric heterochromatin, and nucleolus.
Ki-67 shows cell cycle-dependent distribution: perinucleolar expression in G1, nuclear matrix localization in G2, and chromosomal association during mitosis.
As a proliferation marker, Ki-67 is absent in G0 cells and increases from G1 through mitosis. Ki-67 antibodies are valuable for determining the growth fraction in neoplasms, with prognostic value comparable to thymidine-labeling indices. Low Ki-67 index correlates with histologically low-grade tumors. It is routinely used as a neuronal marker for cell cycling and proliferation.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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