Thermo Fisher Scientific Ki-67 Monoclonal Antibody (MKI67, 2461)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

Ki-67 is a nuclear protein expressed during various stages of the cell cycle, particularly during late G1, S, G2, and M phases. It contains a forkhead-associated (FHA) domain that enables association with euchromatin at the perichromosomal layer, centromeric heterochromatin, and nucleolus.
Ki-67 shows cell cycle-dependent localization: perinucleolar in G1, nuclear matrix in G2, and chromosomal during mitosis. Because Ki-67 is absent in G0 cells and increases from G1 through mitosis, it serves as a reliable proliferation marker.
Ki-67 antibodies are valuable for assessing cell proliferation in neoplasms, correlating with tumor grade and growth fraction, and are widely used as neuronal markers of cell cycling and proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.