Thermo Fisher Scientific Ki-67 (Proliferating Cell Marker) Monoclonal Antibody (MKI67/6571)
인간 Ki-67 단백질을 인식하는 단클론 항체로 세포 증식 마커 연구에 적합. IHC(P) 실험에서 높은 특이성과 재현성을 제공. 단백질 A/G 정제, 액상 형태, 4°C 보관. 세포 주기 관련 연구 및 종양 증식 지표 분석에 활용 가능.
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Thermo Fisher Scientific Ki-67 (Proliferating Cell Marker) Monoclonal Antibody (MKI67/6571)
Applications
- Immunohistochemistry (Paraffin) [IHC (P)]
Tested Dilution: 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MKI67/6571 |
| Immunogen | Recombinant human Ki67 protein fragment (around aa 2293–2478) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive Control: Any actively proliferating cells (e.g., human skin, tonsil, lymph node)
- Cellular Location: Nucleus
Target Information
Ki-67 is a nuclear protein expressed during various stages of the cell cycle, particularly in late G1, S, G2, and M phases. It contains a forkhead-associated (FHA) domain that enables interaction with euchromatin, centromeric heterochromatin, and the nucleolus.
Ki-67 exhibits cell cycle-dependent localization: perinucleolar at G1, nuclear matrix at G2, and chromosomal during M phase.
Because Ki-67 is absent in G0 cells but increases from G1 through mitosis, it serves as a reliable proliferation marker.
Ki-67 antibodies are widely used to determine the growth fraction of neoplasms, showing strong correlation between low Ki-67 index and histologically low-grade tumors.
It is also used as a neuronal marker for cell cycling and proliferation studies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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