Thermo Fisher Scientific HLA-A2 Monoclonal Antibody (BB7.2), PE

Applications

• Flow Cytometry (Flow): Neat

Product Specifications

Additional Formats

• Unconjugated (MA1-80117)
• FITC (MA5-16587)
• Request custom conjugation

Product Specific Information

• Reconstitute with 1 mL of distilled water.
• After reconstitution, store product undiluted at 4°C in the dark.
• For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
• Mouse anti-Human HLA-A2 antibody, clone BB7.2 recognizes the human HLA-A2 histocompatibility antigen.

Target Information

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is membrane-anchored and plays a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. It is expressed in nearly all cells.

The heavy chain (~45 kDa) gene contains 8 exons:

1. Exon 1 encodes the leader peptide
2. Exons 2 and 3 encode the α1 and α2 domains (peptide-binding)
3. Exon 4 encodes the α3 domain
4. Exon 5 encodes the transmembrane region
5. Exons 6 and 7 encode the cytoplasmic tail

Polymorphisms within exons 2 and 3 determine peptide-binding specificity. Typing for these polymorphisms is routinely used in bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described.

HLA and MHC antibodies are essential in Immunopeptidomics, aiding in the identification and characterization of neoantigens via HPLC coupled with tandem mass spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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