Thermo Fisher Scientific V5 Tag Monoclonal Antibody (SV5-Pk1), FITC

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

1:100

-

Product Specifications

Species Reactivity

Tag

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

SV5-Pk1

Immunogen

Paramyxovirus SV5, Simian-Virus 5.

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1% BSA

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_935873

Product Specific Information

MA1-80281 recognizes the sequence IPNPLLGL, present on the P/V proteins of the paramyxovirus, SV5. This antibody has been used to detect recombinant proteins, including epitope-tagged transmembrane and secreted proteins. Successfully used tags include a 14-amino acid tag (gly lys pro ile pro asn pro leu leu gly leu asp ser thr) and a smaller 9-amino acid tag that has been used to inhibit the binding of the monoclonal antibody to its native protein.

Target Information

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.