Thermo Fisher Scientific 5-Methylcytidine Monoclonal Antibody (33D3)
5-Methylcytidine를 인식하는 mouse monoclonal antibody (clone 33D3). 다양한 응용(IHC, ICC, Flow, ELISA, ChIP)에 사용 가능. Protein A로 정제된 고순도 항체로 장기 보관 시 -20°C에서 안정적. DNA 메틸화 연구에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | Assay-dependent |
| ELISA | Assay-dependent |
| ChIP assay (ChIP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 33D3 |
| Immunogen | Proprietary Immunogen |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_926789 |
Product Specific Information
- Heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) is recommended for staining paraffin sections.
- For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
- Mouse anti-5-methylcytidine antibody, clone 33D3 recognizes 5-methylcytidine, a modified base found in the DNA of plants and vertebrates.
Target Information
Modified base 5-methylcytidine (5-MeCyd) is found in DNA of plants and vertebrates. DNA methylation is a modification process involved in gene expression control. Reports suggest that in tumors, DNA is frequently globally hypomethylated compared to DNA from normal tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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