Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), eFluor 506, eBioscience

Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), eFluor 506, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 647, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 563, 661, 737, 805
• Brilliant Violet 421, 650, 711, 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 585, 610-70S, 660-120S, 760
• Pacific Orange
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Rα). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity, but associates with CD122 (IL-2Rβ) and CD132 (common γ chain) to form the high-affinity IL-2 receptor. Binding of IL-2 to both receptor classes is inhibited by the PC61.5 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of normal human peripheral blood cells. Recommended ≤0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration for best performance.

eFluor™ 506 is excited by a 405 nm violet laser and emits at 506 nm. Use a 510/20 band pass filter or equivalent. Ensure instrument compatibility.

Excitation: 405 nm
Emission: 506 nm
Laser: Violet Laser

Target Information

CD25 (IL2 receptor alpha chain, IL2RA) is a cytokine receptor involved in T and B lymphocyte proliferation. The receptor complex consists of IL2RA, IL2RB, and IL2RG chains forming the high-affinity IL-2 receptor. Homodimeric IL2RA or IL2RB chains form low- and medium-affinity receptors, respectively. IL2 expression in mature thymocytes is monoallelic, providing precise regulation. IL2 acts as a growth and differentiation factor and regulates apoptosis. It promotes B cell proliferation, enhances NK cell activity, and inhibits granulocyte macrophage colony formation. Disruption of this gene in mice leads to ulcerative colitis-like disease, indicating its role in immune response regulation. Mutations are associated with IL-2 receptor alpha deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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