Thermo Fisher Scientific CD20 Monoclonal Antibody (2H7), eFluor 506, eBioscience
인간 CD20을 인식하는 2H7 클론 단클론 항체로, eFluor 506 형광체가 결합된 제품입니다. Flow cytometry에 최적화되어 있으며, 405 nm 레이저로 여기되어 506 nm에서 방출합니다. 친화 크로마토그래피로 정제된 액상 형태이며, B세포 표지 및 활성화 연구용으로 적합합니다.
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Thermo Fisher Scientific CD20 Monoclonal Antibody (2H7), eFluor 506, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 4 publications |
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test | View 11 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2H7 |
| Conjugate | eFluor™ 506 |
| Excitation / Emission Max | 419 / 508 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2637144 |
Available Formats
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 615 / 737 / 805
- Brilliant Violet 421 / 605 / 650 / 711
- eFluor 450
- FITC
- NovaFluor Blue 610-70S / 660-120S
- NovaFluor Red 710 / 725
- NovaFluor Yellow 590 / 660
- PE / PE-Cyanine5 / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436 / 600 / 645 / 702 / 780
- Request custom conjugation available
Product Specific Information
The 2H7 monoclonal antibody reacts with human CD20, a 33–36 kDa transmembrane protein expressed on developing and mature B cells but not plasma cells. Low levels may be found on a subset of mature T cells. CD20 is involved in B-cell activation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-titrated and tested on normal human peripheral blood cells
- Recommended Usage: 5 µL (0.5 µg) per test (100 µL final volume)
- Cell Range: 10⁵–10⁸ cells/test (empirically determined)
- Fluorochrome: eFluor® 506 (Excitation: 405 nm; Emission: 506 nm)
- Recommended Filter: 510/20 band pass or equivalent
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD20 is a non-glycosylated surface phosphoprotein (33–37 kDa) expressed on mature and malignant B cells, some T lymphocytes, and follicular dendritic cells. It regulates transmembrane calcium conductance, cell cycle progression, and B-cell proliferation. CD20 is associated with lipid rafts and plays a role in BCR aggregation and signaling. It remains on the cell surface after antibody binding, making it an effective therapeutic target for B-cell depletion.
Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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