Thermo Fisher Scientific Histone H4 Recombinant Rabbit Monoclonal Antibody (SD209-04)
Histone H4 단백질을 표적하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC/IF, ChIP 등 다양한 응용에 적합합니다. 높은 특이성과 재현성을 제공하며, Protein A로 정제된 액상 항체입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ChIP assay (ChIP) | 0.5–2 µg |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SD209-04 |
| Immunogen | Synthetic peptide within C-terminal Human Histone H4 (aa 54–103) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809651 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fibers in eukaryotes. Two molecules each of H2A, H2B, H3, and H4 form an octamer, around which approximately 146 bp of DNA is wrapped to form nucleosomes. The linker histone H1 interacts with linker DNA between nucleosomes and contributes to chromatin compaction.
The Histone H4 gene is intronless, encodes a member of the histone H4 family, and is located in the large histone gene cluster on chromosome 6. Transcripts lack polyA tails but contain a palindromic termination element.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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