Thermo Fisher Scientific betacellulin Polyclonal Antibody, PeproTech
Rabbit polyclonal antibody specific to human Betacellulin. Validated for WB and ELISA with high sensitivity. Produced from E.coli-derived recombinant antigen and purified by antigen affinity chromatography. Suitable for research use only.
- 카탈로그번호
- 500-P254-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific betacellulin Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.5–2.0 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human Betacellulin |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930085 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
DGNSTRSPET NGLLCGDPEE NCAATTTQSK RKGHFSRCPK QYKHYCIKGR CRFVVAEQTP SCVCDEGYIG ARCERVDLFY
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human Betacellulin. Anti-Human Betacellulin-specific antibody was purified by affinity chromatography employing an immobilized Human Betacellulin matrix.
Sandwich ELISA:
To detect Human Betacellulin by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Betacellulin (500-P254Bt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human Betacellulin.
Western Blot:
To detect Human Betacellulin by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human Betacellulin is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Target Information
The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is processed to the mature molecule by proteolytic events. This protein is a ligand for the EGF receptor.
For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.
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