Thermo Fisher Scientific Connexin 45 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:10,000

-

Immunocytochemistry (ICC/IF)

1:10,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Sheep / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues Q(354) A Y S H Q N N P H G P R E(367) of human Connexin-45. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Connexin 45,uniProtId:P36383-1,ncbiNodeId:9606,antigenRange:354-367,antigenLength:396,antigenImageFileName:PA1-18408_Connexin_45_P36383-1_Sheep.svg,antigenImageFileNamePDP:PA1-18408_Connexin_45_P36383-1_Sheep_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2111176

Product Specific Information

Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20 °C for a higher stability, and at 4 °C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

Target Information

Gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell closely packed transmembrane channels. Proteins, called connexins, purified from fractions of enriched gap junctions from different tissues differ. Connexins are designated by their molecular mass. Another system of nomenclature divides gap junction proteins into 2 categories, alpha and beta, according to sequence similarities at the nucleotide and amino acid levels. For example, CX43 is designated alpha-1 gap junction protein, whereas CX32 and CX26 are called beta-1 and beta-2 gap junction proteins, respectively. This nomenclature emphasizes that CX32 and CX26 are more homologous to each other than either of them is to CX43. Connexins have four transmembrane, three intracellular, and two extracellular regions. Different tissues express different connexins, though tissue specificities overlap, and a given tissue or cell can express several different connexins. Developmental regulation of at least some of the connexin genes has been found. Embryo implantation is regulated in part by temporally changing patterns of expression of connexins in the embryo and the maternal decidua.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.