Thermo Fisher Scientific BUBR1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

-

Immunocytochemistry (ICC/IF)

2 µg/mL

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Protein corresponding to Human BUB1B (aa 623-818) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:BUBR1,uniProtId:O60566-1,ncbiNodeId:9606,antigenRange:623-818,antigenLength:1050,antigenImageFileName:720297_BUBR1_O60566-1_Rabbit.svg,antigenImageFileNamePDP:720297_BUBR1_O60566-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2610165

Product Specific Information

These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.

This antibody is predicted to react with Monkey, Horse and Pig.

Target Information

BUBR1 is a member of the Ser/Thr protein kinase family. It is a component of the mitotic checkpoint that delays anaphase until all chromosome are properly attached to the mitotic spindle. Compromised spindle checkpoints are thought to play an important role in genetic instability that predisposes cells to malignant transformations. Human cells contain two related checkpoint protein kinases, evolved from the BUB1 gene; hBUBR1 and hBUB1. They are important components of the spindle checkpoint Both kinases monitor mitotic kinetochore-microtubule interactions. hBUBR1 is essential for normal mitotic progression as it prevents cells from entering into anaphase too early. BUBR1 has been detected in human cancers. It has been identified as a novel binding partner of BCSG1 (Breast Cancer-Specific Gene 1), which has been suggested to accelerate the progression of breast cancer by compromising the mitotic checkpoint control through inactivation of BUBR1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.