Thermo Fisher Scientific 6x His Tag Recombinant Mouse Monoclonal Antibody (8-F0-R)
6x His 태그 단백질 검출용 마우스 단클론 항체로 Western blot과 IP에 적합. HEK293 세포 발현, Protein A 정제, 1 mg/mL 농도. 비결합형 형태로 안정적이며, His-tag 단백질의 정제 및 검출에 활용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:5,000–1:20,000
Immunoprecipitation (IP)
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgG2a |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 8-F0-R |
| Immunogen | C-terminal His-tagged recombinant protein, N-terminal His-tagged recombinant protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 0.1% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_3090459 |
Product Specific Information
Positive control: C-terminal His-tag fusion protein lysates.
Target Information
The 6x His tag is a synthetic oligo peptide consisting of six consecutive histidine residues (HHHHHH).
This tag is commonly expressed at either the N- or C-terminal regions of recombinant proteins to enable isolation or purification by immobilized metal affinity chromatography (IMAC).
Epitope tagging allows detection of recombinant proteins lacking specific antibodies by fusing a known epitope to the protein.
His-tagged proteins can be purified using Ni²⁺-NTA affinity chromatography due to the strong and selective binding of histidine clusters to nickel ions.
His tag-specific antibodies facilitate detection or co-immunoprecipitation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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