Thermo Fisher Scientific 6x His Tag Recombinant Mouse Monoclonal Antibody (A5D1-R)
6x His 태그 단백질 검출용 재조합 마우스 단일클론 항체. Western blot 및 면역침강(IP)에 적합. 높은 특이성과 재현성을 제공하며, HEK293 세포에서 발현된 IgG1 형 항체. 연구용으로만 사용 가능.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific 6x His Tag Recombinant Mouse Monoclonal Antibody (A5D1-R)
Thermo Fisher Scientific 6x His Tag Recombinant Mouse Monoclonal Antibody (A5D1-R)
Applications
- Western Blot (WB): 1:1,000–1:50,000
- Immunoprecipitation (IP): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgG1 |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | A5D1-R |
| Immunogen | Synthetic peptide HHHHHHGCGHHHHHH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 0.1% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3090458 |
Product Specific Information
Positive control: C-terminal His-tag fusion protein lysates, Mid His-tag fusion protein lysates.
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH).
It is commonly expressed at either the N- or C-terminal region of recombinant proteins to enable isolation or purification via immobilized metal affinity chromatography (IMAC).
Epitope tagging allows detection of recombinant proteins by fusing a known epitope to the target protein.
His-tagged proteins can be purified using metal chelate chromatography, leveraging the strong and selective affinity of histidine clusters for Ni²⁺ ions, enabling purification to homogeneity on Ni²⁺-NTA adsorbents.
His tag-specific antibodies facilitate detection or co-immunoprecipitation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.