Thermo Fisher Scientific DYKDDDDK Tag Recombinant Mouse Monoclonal Antibody (PS00-M2)
DYKDDDDK(FALG) 태그 단백질 검출용 재조합 마우스 단일클론 항체. WB, ICC/IF, IP 등 다양한 응용에 최적화. 높은 특이성과 재현성을 제공하며, HEK293 세포 발현 시스템 기반. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:25,000 |
| Immunocytochemistry (ICC/IF) | 1:250 |
| Immunoprecipitation (IP) | 1:1,000–1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgG1 |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | PS00-M2 |
| Immunogen | Synthetic peptide immune sequence is N-DYKDDDDK-C |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 0.1% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_3090457 |
Product Specific Information
Positive control: C-terminal FLAG-tag fusion protein lysate, N-terminal FLAG-tag fusion protein lysate.
Target Information
DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence widely used as a protein tag to facilitate protein purification, detection, and localization.
The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys.
It is commonly fused to the N- or C-terminus of a protein of interest, enabling easy identification and isolation using anti-FLAG antibodies.
The DYKDDDDK tag is utilized in molecular biology and biochemistry research for protein-protein interaction studies, expression analysis, and localization studies.
Its hydrophilic nature minimizes protein denaturation, ensuring compatibility with tagged proteins.
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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