Thermo Fisher Scientific DYKDDDDK Tag Recombinant Mouse Monoclonal Antibody (PS00-M2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:25,000

-

Immunocytochemistry (ICC/IF)

1:250

-

Immunoprecipitation (IP)

1:1,000-1:10,000

-

Product Specifications

Species Reactivity

Tag

Host/Isotype

Mouse / IgG1

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

PS00-M2

Immunogen

Synthetic peptide immune sequence is N-DYKDDDDK-C.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3090457

Product Specific Information

Positive control: C-terminal FLAG-tag fusion protein lysate, N-terminal FLAG-tag fusion protein lysate.

Target Information

DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.