Thermo Fisher Scientific Myc Tag Recombinant Mouse Monoclonal Antibody (A3C8-R-2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:10,000

-

ELISA (ELISA)

1:10,000

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Tag

Host/Isotype

Mouse / IgG1

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

A3C8-R-2

Immunogen

Synthetic peptide EQKLISEEDL conjugated to keyhole limpet hemocyanin.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3092412

Product Specific Information

Positive control: C-terminal Myc-tag fusion protein lysate, N-terminal Myc-tag fusion protein lysate.

Product MA5-50639 was used in an Indirect ELISA.

Target Information

Myc is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.