Thermo Fisher Scientific PDK1 Recombinant Rabbit Monoclonal Antibody (JA67-30)
Recombinant rabbit monoclonal antibody recognizing PDK1 with high specificity and sensitivity. Validated for WB, IHC, ICC/IF, and Flow Cytometry. Provides consistent performance and animal-origin-free formulation for reliable research use.
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Thermo Fisher Scientific PDK1 Recombinant Rabbit Monoclonal Antibody (JA67-30)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:5000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:2000 | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | - |
| Flow Cytometry (Flow) | 1:50–1:100 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JA67-30 |
| Immunogen | Synthetic peptide within Human PDK1 aa 16–65 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809979 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates.
Key advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
PDK1 (3-phosphoinositide dependent kinase-1) is a serine/threonine protein kinase localized to the plasma membrane. It plays a vital role in insulin-induced glycogen synthesis, PKC activation, and activation of the AGC subfamily of protein kinases.
PDK1 is crucial for cellular responses to hypoxia, supports cell proliferation under hypoxic conditions, and protects cells against apoptosis induced by hypoxia and oxidative stress.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일: MA5-32702_PDK1_Q15118-1_Rabbit.svg, MA5-32702_PDK1_Q15118-1_Rabbit_PDP.jpeg)
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