Thermo Fisher Scientific PDK1 Recombinant Rabbit Monoclonal Antibody (JA67-30)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:2,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:50-1:200

-

Flow Cytometry (Flow)

1:50-1:100

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JA67-30

Immunogen

Synthetic peptide within Human PDK1 aa 16-65 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PDK1,uniProtId:Q15118-1,ncbiNodeId:9606,antigenRange:16-65,antigenLength:436,antigenImageFileName:MA5-32702_PDK1_Q15118-1_Rabbit.svg,antigenImageFileNamePDP:MA5-32702_PDK1_Q15118-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2809979

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

PDK1 (3-phosphoinositide dependent kinase-1) is a serine/threonine protein kinase localized to the plasma membrane. It plays a vital role in insulin-induced glycogen synthesis, PKC activation, and activation of the AGC subfamily of protein kinases. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.