Thermo Fisher Scientific BAG3 Recombinant Rabbit Monoclonal Antibody (JA90-53)

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000–1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200

Product Specifications

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, followed by screening to select optimal clones for production.
Advantages include:

• Improved specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulation
• Broader immunoreactivity due to larger rabbit immune repertoire

Target Information

BAG3 is one of the BAG protein family members that compete with Hip for binding to the Hsc70/Hsp70 ATPase domain, promoting substrate release.
All BAG proteins contain an approximately 45-amino acid BAG domain near the C-terminus but differ in their N-terminal regions.
The BAG3 gene encodes a protein with a WW domain at the N-terminal region and a BAG domain at the C-terminal region.
BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells, binding with high affinity and inhibiting chaperone activity in a Hip-repressible manner.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.