Thermo Fisher Scientific CD272 (BTLA) Monoclonal Antibody (6G3), eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

6G3

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_467752

Product Specific Information

Description: The 3F9.D12 antibody reacts with mouse BTLA, B and T lymphocyte attenuator from both BALB/c and C57Bl/6 strains. BTLA is expressed by peripheral lymphocytes, splenic macrophages, developing B cells in the bone marrow and developing T cells in thymus and mature, but not immature bone marrow-derived dendritic cells. BTLA is implicated as a negative regulator of the activation and/or function of various hemopoietic cell types. It is reported that BTLA binds to B7-H4, but further studies are needed to confirm this interaction.

Note: The anti-mouse BTLA monoclonal antibody 6F7 is reported to stain CD4+ and CD8+ single-positive (SP) thymocytes (Hurchla et al). However, other anti-mouse BTLA clones generated simultaneously with 6F7 (8F4, 3F9.D12, 6G3 and 6H6) do not stain SP thymocytes. It is not understood why there is a discrepancy in thymocyte staining however clones 8F4, 3F9.D12, 6G3 and 6H6 stain similar populations to 6F7 in splenocytes and bone marrow cells.

Applications Reported: This 6G3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 6G3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

BTLA or B and T-lymphocyte attenuator is a member of the co-inhibitory receptors of the CD28 superfamily. BTLA is a lymphocyte inhibitory receptor which inhibits lymphocytes during immunes responses. BTLA is constitutively expressed on most CD4+ and CD8+ T cells and its expression progressively decreases upon T cell activation. It remains expressed on Th1 cells, but not Th2 cells. BTLA is a unique co-receptor that interacts with the tumor necrosis factor receptor superfamily member herpesvirus entry mediator (HVEM). This interation is an important pathway regulating lymphocyte activation and/or homeostasis in the immune response.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.