Thermo Fisher Scientific AID Monoclonal Antibody (mAID-2), eBioscience

Thermo Fisher Scientific AID Monoclonal Antibody (mAID-2), eBioscience

Applications and Tested Dilutions

Product Specifications

Additional Formats:

• Biotin
• Request custom conjugation

Product Specific Information

The monoclonal antibody mAID-2 recognizes human and mouse AID (Activation-Induced Cytidine Deaminase, gene AICDA). AID is a 24 kDa RNA-editing enzyme that converts cytosine into uracil, playing a critical role in B cell development through somatic hypermutation (SHM) and immunoglobulin class switch recombination (CSR).

Altered AID expression levels are associated with disease states. Mutations in AID cause Hyper-IgM Syndrome type 2, while overexpression correlates with poor prognosis in chronic lymphocytic leukemia/small lymphocytic lymphoma.

AID is expressed mainly by activated mature B cells, including germinal center (GC) B cells and intermediate GC cells (IgD+CD38-CD23-CD71+). It is also found in hepatocellular carcinoma, gastric epithelial cells infected with Helicobacter, oocytes, and embryonic stem cells. Due to its nuclear localization signal (NLS), expression occurs both in the nucleus and cytoplasm.

Applications Reported:

• Immunoblotting (WB)
• Immunohistologic staining of frozen tissue sections
• Immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections

Applications Tested:
Tested by western blot and/or IHC on FFPE sections using low or high pH antigen retrieval. Recommended concentration ≤ 20 µg/mL. Antibody should be titrated for optimal assay performance.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

This gene encodes an RNA-editing deaminase, a member of the cytidine deaminase family. It is involved in somatic hypermutation, gene conversion, and class-switch recombination of immunoglobulin genes. Defects in this gene cause autosomal recessive hyper-IgM immunodeficiency syndrome type 2 (HIGM2).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.