Thermo Fisher Scientific AID Monoclonal Antibody (mAID-2), eBioscience
AID 단백질을 인식하는 mAID-2 단클론 항체로, 인간 및 생쥐 시료에 반응합니다. Western blot, IHC, ICC 등 다양한 응용 가능하며, 0.5 mg/mL 농도의 액상 형태로 제공됩니다. 높은 순도(>90%)와 낮은 응집도(<10%)로 연구용에 적합합니다.
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Thermo Fisher Scientific AID Monoclonal Antibody (mAID-2), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 20 µg/mL | 11 publications |
| Immunohistochemistry (IHC) | - | 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 20 µg/mL | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 20 µg/mL | - |
| Immunocytochemistry (ICC/IF) | - | 2 publications |
| Flow Cytometry (Flow) | - | 2 publications |
| Immunoprecipitation (IP) | - | 1 publication |
| ChIP assay (ChIP) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | mAID-2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865181 |
Additional Formats:
Product Specific Information
The monoclonal antibody mAID-2 recognizes human and mouse AID (Activation-Induced Cytidine Deaminase, gene AICDA). AID is a 24 kDa RNA-editing enzyme that converts cytosine into uracil, playing a critical role in B cell development through somatic hypermutation (SHM) and immunoglobulin class switch recombination (CSR).
Altered AID expression levels are associated with disease states. Mutations in AID cause Hyper-IgM Syndrome type 2, while overexpression correlates with poor prognosis in chronic lymphocytic leukemia/small lymphocytic lymphoma.
AID is expressed mainly by activated mature B cells, including germinal center (GC) B cells and intermediate GC cells (IgD+CD38-CD23-CD71+). It is also found in hepatocellular carcinoma, gastric epithelial cells infected with Helicobacter, oocytes, and embryonic stem cells. Due to its nuclear localization signal (NLS), expression occurs both in the nucleus and cytoplasm.
Applications Reported:
- Immunoblotting (WB)
- Immunohistologic staining of frozen tissue sections
- Immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections
Applications Tested:
Tested by western blot and/or IHC on FFPE sections using low or high pH antigen retrieval. Recommended concentration ≤ 20 µg/mL. Antibody should be titrated for optimal assay performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
This gene encodes an RNA-editing deaminase, a member of the cytidine deaminase family. It is involved in somatic hypermutation, gene conversion, and class-switch recombination of immunoglobulin genes. Defects in this gene cause autosomal recessive hyper-IgM immunodeficiency syndrome type 2 (HIGM2).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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