Thermo Fisher Scientific SOX10 Monoclonal Antibody (20B7), eBioscience
SOX10 단백질을 인식하는 단클론 항체(20B7)로 인간, 생쥐, 랫트 반응성. WB, IHC, ICC 등 다양한 응용 가능. 신경 및 멜라닌세포 발달 연구에 적합. 고순도(>90%) 및 0.2 µm 여과 처리된 액상 형태.
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Thermo Fisher Scientific SOX10 Monoclonal Antibody (20B7), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 10 µg/mL | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 10 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 5–10 µg/mL | - |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 20B7 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2572891 |
Product Specific Information
The monoclonal antibody 20B7 recognizes Sox10, a member of the Sox superfamily (SRY-related HMG-box) containing a DNA-binding high-mobility group domain and functioning as a transcription factor. Sox10 expression is initially localized to the developing neural crest and peripheral nervous system, later appearing in glial progenitors and mature oligodendrocytes. It plays roles in stem cell maintenance, melanocyte differentiation, and both central and peripheral gliogenesis and neurogenesis.
Sox10 regulates downstream proteins such as MASH1, PHOX2B, PDGF-alpha, and MITF in an expression-dependent manner. It is also expressed in multipotent vascular stem cells, melanomas, and gliomas.
The 20B7 antibody recognizes human and rat Sox10, and based on homology, should also recognize mouse Sox10.
Applications Reported:
- Immunohistochemical staining of frozen and formalin-fixed paraffin-embedded tissue sections
- Microscopy
- Immunocytochemistry
Applications Tested:
- Immunocytochemistry on formaldehyde-fixed and permeabilized rat cells
- Immunohistochemistry on human formalin-fixed paraffin-embedded tissue sections using low pH antigen retrieval
- Recommended concentration ≤10 µg/mL for both applications
- Titrate for optimal performance in specific assays
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
SOX10 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in embryonic development and cell fate determination. It may act as a transcriptional activator after forming complexes with other proteins and functions as a nucleocytoplasmic shuttle protein essential for neural crest and peripheral nervous system development. Mutations in SOX10 are associated with Waardenburg-Shah and Waardenburg-Hirschsprung diseases.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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