Thermo Fisher Scientific SOX10 Monoclonal Antibody (20B7), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/ml

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL

-

Immunohistochemistry (Frozen) (IHC (F))

10 µg/mL

-

Immunocytochemistry (ICC/IF)

5 - 10 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

20B7

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572891

Product Specific Information

Description: The monoclonal antibody 20B7 recognizes Sox10, is a member of the Sox superfamily (SRY-related HMG-box) that contain a DNA binding domain (high-mobility group domain) and functions as a transcription factor. Initially, Sox10 expression is localized to the developing neural crest and peripheral nervous system, but later is expressed in glial progenitors and mature oligodendrocytes. Sox10 is involved in stem cell maintenance, melanocyte differentiation, as well as central and peripheral gliogenesis and neurogenesis. Sox10 regulation of downstream proteins such as MASH1, PHOX2B, PDGF-alpha, and MITF is dependent on expression level. Sox10 is also expressed in multipotent vascular stem cells, melanomas, and gliomas.

The 20B7 antibody is reported to recognize human and rat Sox 10, and based on homology should recognize mouse Sox10.

Applications Reported: This 20B7 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.

Applications Tested: This 20B7 antibody has been tested by immunocytochemistry of formaldehyde fixed and permeabilized rat cells. This 20B7 antibody has also been tested by immunohistochemistry of human formalin-fixed paraffin embedded tissue sections using low pH antigen retreival and can be used at less than or equal to 10 µg/mL for both applications. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

SOX10 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. This protein may act as a transcriptional activator after forming a protein complex with other proteins. It acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.