Thermo Fisher Scientific iNOS Monoclonal Antibody (CXNFT), eBioscience

Thermo Fisher Scientific iNOS Monoclonal Antibody (CXNFT), eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugated Formats

• Alexa Fluor 488
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 395 / 563 / 615 / 737
• Brilliant Violet 421 / 480 / 605 / 786
• eFluor 450
• PE / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Custom conjugation available upon request

Product Specific Information

This CXNFT monoclonal antibody reacts to mouse NOS2 (inducible NOS, iNOS). Nitric oxide synthase enzymes catalyze the formation of nitric oxide from L-arginine through an NADPH- and oxygen-dependent mechanism.
NOS1 (nNOS) and NOS3 (eNOS) are constitutively expressed, while NOS2 is induced by bacterial endotoxins and inflammatory cytokines such as IFN-γ and TNF-α.
NOS2 is expressed in myeloid-derived suppressor cells and M1 macrophages, but not in M2 macrophages. NOS enzymes are active as homodimers, and NOS2 becomes functionally active upon dimerization at physiological Ca²⁺ levels.

Applications Reported:

• Intracellular staining followed by flow cytometric analysis
• Western blotting (fluorochrome-conjugated CXNFT recommended for flow cytometry)

Applications Tested:

• Immunoblot analysis of LPS-stimulated mouse peritoneal exudate cells (5 µg/mL)
• Intracellular staining and flow cytometric analysis of NOS2-transfected HEK-293T cells (≤0.25 µg/test)
• Optimal antibody titration recommended for assay performance

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

iNOS (Inducible Nitric Oxide Synthase):
iNOS produces nitric oxide (NO), a gaseous free radical involved in vasorelaxation, neurotransmission, and cytotoxicity.
NO synthesis is mediated by NOS family enzymes (NOS1, NOS2, NOS3). iNOS is inducible by lipopolysaccharide and cytokines (IFN-γ, TNF, IL-1, IL-2).
It is expressed in macrophages, hepatocytes, synoviocytes, and smooth muscle cells.
After cytokine induction, iNOS activity increases significantly over time, leading to sustained NO production.
Three iNOS pseudogenes are located on chromosome 17 (Smith-Magenis syndrome region).
Dysfunction of iNOS is associated with diseases such as achalasia and impotence.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.